The ceremony took place at the 31st Tactical Air Base in Poznań-Krzesiny, west-central Poland, where the first Polish F-16s arrived in November 2006.
The country now has 47 of the jets, known in Polish service as the Jastrząb (Hawk).
They are based in Poznań-Krzesiny and at Łask, central Poland.
Major General Tomasz Jatczak, inspector of the Polish Air Force, called the F-16s and the people who fly and maintain them "the foundation of our air defence."
He told reporters the aircraft still had plenty of service life and potential for upgrades.
A Mid-Life Upgrade of the jets, including their onboard radars and avionics, is due to begin in 2029.
He said the work would be spread over time to balance sending aircraft away against training and operational readiness.
Brigadier General Norbert Chojnacki said the F-16 had become a symbol of Poland's place in NATO and its ability to work with allies.
Joint exercises and missions had given Polish pilots a level of training they could "proud of," he added.
In a letter read at the ceremony, Deputy Prime Minister and Defence Minister Władysław Kosiniak-Kamysz said. Polish military aviation had "entered a new era" with the F-16.
It was not only about buying modern equipment, he noted, but above all about introducing new standards of training, logistics and tactical procedures.
The F-16s will be succeeded by fifth-generation F-35s, made by US firm Lockheed Martin.
Poland signed a USD 4.6 billion contract for 32 of them in early 2020; the first arrived in May and deliveries are due to be completed in 2029.
(ał)
Source: PAP