Among those freed was Katsyaryna Andreyeva, a journalist with Polish Belarusian-language broadcaster Belsat, according to reports.
Also released was Marfa Rabkova, an activist with Belarusian human rights group Viasna who was arrested in September 2020 and sentenced to 14 years and nine months on charges including "extremism," the Reuters news agency reported.
Bloggers and protesters were also among those released following talks in Minsk between Lukashenko and John Coale, an envoy for US President Donald Trump.
Prior to Thursday's announcement, more than 1,100 political prisoners were being held in Belarus, according to Viasna.
Coale said he expected all remaining political prisoners to be released by the end of the year, adding that Washington would lift all sanctions imposed over the 2020 crackdown on protesters if that happens, Reuters reported.
He also said Lukashenko had been urged to halt further arrests.
The United States has agreed to lift sanctions on several Belarusian banks and the country’s finance ministry, as well as fertiliser producers, according to the AFP news agency.
Coale said earlier on Thursday that Lukashenko may soon visit the United States, a trip that would signal a potential breakthrough after years of diplomatic isolation over human rights abuses and his support for Russia's invasion of Ukraine.
Previous talks between Coale and Lukashenko led to the release of dozens of political prisoners in September and a further 123 in December, including Nobel Peace Prize winner Ales Bialiatski.
In response, the United States lifted sanctions on Belarusian potash, a key export used in fertilisers.
Exiled Belarusian opposition leader Sviatlana Tsikhanouskaya welcomed the latest prisoner release as a moment of "great joy" and "huge relief.”
She expressed gratitude to Trump and his administration, but said that "many people are still behind bars."
Among them is Andrzej Poczobut, a Polish-Belarusian journalist imprisoned in Belarus since 2021, who is serving an eight-year sentence imposed by the Lukashenko regime, Polish media reported.
"Our goal remains unchanged — to free them all and to put an end to repression, so that every Belarusian can live freely in their own country," Tsikhanouskaya said on X.