In an interview with the newspaper, Trump described the alliance as a "paper tiger" and said leaving the military alliance was now "beyond reconsideration."
“Oh yes, I would say [it’s] beyond reconsideration,” Trump said when asked whether he would rethink US membership after the Iran war.
“I was never swayed by NATO. I always knew they were a paper tiger, and Putin knows that too,” he added.
Trump has long expressed scepticism about NATO and its effectiveness, frequently criticising allies over defence spending and burden-sharing.
Earlier this month, he hit out at NATO allies for not supporting the US-Israel war against Iran, calling them “cowards” in a social media post.
"COWARDS, and we will REMEMBER!" he wrote, using capital letters for emphasis.
He complained that allies had refused to join efforts to secure shipping through the Strait of Hormuz, which Iran has blocked. About 20 percent of the world’s oil supply passes through the strait.
Most US allies were not consulted ahead of the military operation, according to the Reuters news agency.
The conflict, which began with US and Israeli strikes on Iran on February 28, has roiled global markets, killed thousands and displaced millions, it reported.
In an earlier comment, Trump said most NATO allies had told Washington they did not want to get involved in the military operation, calling their stance a "very foolish mistake."
In comments to the Financial Times in mid-March, Trump warned NATO could face a "very bad future" if allies do not respond to his call to help secure the Strait of Hormuz.
The Strait of Hormuz is a vital global shipping route linking the Persian Gulf with international markets.
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Source: Reuters, IAR, PAP